Nucleic Acids Isolation Methods


Edited by
Botho Bowien
Institute for Microbiology and Genetics, University of Göttingen, GERMANY
Peter Dürre
Department of Microbiology and Biotechnology, University of Ulm, GERMANY

    April 2004
ca.350 pages, Hardcover
ISBN: 1-58883 - 027- 6

Nucleic Acids Isolation Methods is part of a book series entitled Genome Science and Nucleic Acids. The volume Nucleic Acids Isolation Methods is devoted to preparation of genomic DNA, plasmid DNA, viral nucleic acids, and RNA. Emphasis is also put on stabilization of nucleic acids prior to isolation, scale-up of plasmid isolation, and DNA cleanup from gels, PCR, sequencing, etc. A number of specific medical and forensic applications are detailed such as preparation of pathogen nucleic acids for real-time PCR analysis, extraction of DNA from paraffin embedded bone marrow trephine biopsies, and isolation of nucleic acids from tissue, blood, semen, hair, and saliva for DNA fingerprints, RFLP, and PCR analyses. This book contains 10 state-of-the-art review chapters written by 20 renowned experts from leading research institutions, industry and a criminal investigation department. Detailed description of methods as well as their theoretical background makes this volume an essential reference for specialists in academia, research institutions, and industry and also a valuable manual for newcomers to this field.

  • Stabilization of Nucleic Acids Prior to Isolation, Douglas J. McGarvey
  • Isolation of Genomic DNA, Emma Duncan, Edgar Setzke, and Janina Lehmann
  • Isolation of Plasmid DNA, Kate E. Bendall, Emma Duncan, Eike Griess, and Dirk Schacht
  • Considerations for the Scale up of Plasmid DNA Purification, Markus Müller
  • Purification of Viral Nucleic Acids, Kate E. Bendall, Edgar Setzke, and Hans-Joachim Quandt
  • Isolation of RNA, Douglas J. McGarvey, Hans-Joachim Quandt, Janina Lehmann,
    and Susanne Ullmann

  • DNA Cleanup from Gels, PCR, Sequencing, and Others, Julian Phillips and Ulla Deutsch
  • Nucleic Acids of Pathogens from Biological Samples for Real-time PCR Analysis, Guido Krupp,
    Thomas Laue, Rainer Söller, Thomas Grewing, and Ulrich Spengler

  • Extraction of DNA from Paraffin Embedded Bone Marrow Trephine Biopsies,
    Caroline L. Wickham
  • DNA and Forensic Applications, Anja Gockel, and Peter Berschick
  • Most up-to-date reference summarizing latest methods on nucleic acids isolation methods.
  • Covers nucleic acids isolation methods and their biochemical, biological, forensic and medical applications.
  • 10 state-of-the-art chapters written by research experts and market leaders involved in the production of commercial kits for nucleic acid isolation and PCR diagnostics.
  • Hundreds of bibliographic citations, illustrations, figures, tables and equations.
  • A perfect reference source for students, researchers and specialists working in the fields of nucleic acids, microbiology, biotechnology, genetics, genome science and engineering, biochemistry, medical sciences, biology, biomedical engineering and forensic science.

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